Hospitals are recognized for his or her strict hygiene protocols, but infections acquired in these settings stay a priority. One vital issue is the cleanliness of surfaces, which might harbor dangerous micro organism. Poor sanitation in healthcare services can result in vital well being dangers, together with the unfold of multidrug-resistant micro organism. Making certain that surfaces are successfully decontaminated is subsequently, essential to stopping hospital acquired infections. To handle this concern, an analysis of the effectiveness of present decontamination methods was carried out, specializing in figuring out the bacterial populations current on surfaces earlier than and after cleansing and disinfection, in addition to assessing the susceptibility of those micro organism to 2 disinfectants.
Analysis on the “Université des Montagnes” Instructing Hospital (UdMTH) has demonstrated vital progress in floor decontamination, enhancing hospital hygiene and lowering the danger of healthcare-associated infections. Entitled “Floor decontamination effectiveness on the Université des Montagnes Instructing Hospital: Monitoring within the biomedical evaluation laboratory”, this mission, led by O’Neal Dorsel Youté, Christelle Domngang Noche, Esther Guladys Kougang, and Pierre René Fotsing Kwetche from the Université des Montagnes, Cameroon, together with Blandine Pulchérie Tamatcho Kweyang from the College of Yaoundé I, Cameroon, was carried out to judge the effectiveness of a decontamination protocol within the UdMTH biomedical evaluation laboratory. The findings have been printed within the peer-reviewed journal Heliyon.
The decontamination course of concerned a two-step protocol: cleansing with the detergent “Pax lemon” adopted by disinfection utilizing both Surfanios® or sodium hypochlorite. The staff from Cameroon sought to find out the bacterial populations current on surfaces earlier than cleansing, between cleansing and disinfection and after disinfection and to evaluate the susceptibility of those micro organism to generally used disinfectants.
Earlier than decontamination, excessive bacterial hundreds have been recorded, with Staphylococcus species dominating. The cleansing step alone successfully decreased these bacterial hundreds to values beneath detectable ranges, and after full decontamination, no micro organism have been discovered on the goal surfaces. The staff emphasised, “Excessive bacterial hundreds recorded on these surfaces earlier than decontamination turned undetectable after cleansing with the detergent ‘Pax lemon’.”
The outcomes highlighted that just about all bacterial isolates have been prone to each Surfanios® and sodium hypochlorite, demonstrating the disinfectants’ effectiveness. These authors identified, “Total, these findings indicated course of effectiveness on the subjected bacterial populations and recommend the usage of both Surfanios® or sodium hypochlorite for work surfaces hygiene.”
By way of methodology, the researchers used a descriptive cross-sectional design. Sampling concerned a moist swab technique. The specimens have been collected and analyzed utilizing, for the bacterial detection and enumeration, an adjusted analyze procotol which took into consideration the character of the floor materials and customary bacterial detection protocols, figuring out and characterizing bacterial isolates by way of macroscopy, microscopy, and numerous biochemical assessments. Additionally, micro organism susceptibility assessments to disinfectants have been carried out utilizing adjusted analyze procotol.
The excellent strategy underscores the significance of sustaining rigorous decontamination protocols in healthcare settings. In reference to useful resource availability and affordability related to sodium hypochlorite, the staff concluded that cleansing with the detergent ‘Pax lemon’ and disinfection with sodium hypochlorite could also be enough for the varieties of surfaces subjected of their analysis.
The researchers supplied additional factors to contemplate for the analysis transferring ahead. They recommended that, for environmental contamination or hygiene monitoring, it will be helpful to establish contextual microbial biomarkers that can be utilized affordably in resource-limited contexts. Based mostly on their outcomes and others they’ve used, they’ve proposed the genus Staphylococcus. Additionally, in step with the methodology and their outcomes, one other perspective was to conduct work to enhance the bacterial detection thresholds on surfaces achieved with protocols utilizing moist swab sampling and bacterial tradition strategies.
Youté and his colleagues imagine these findings are essential for bettering hospital hygiene practices not solely at UdMTH however doubtlessly at different healthcare services as nicely. The profitable discount of bacterial hundreds to undetectable ranges emphasizes the effectiveness of the examined decontamination protocols, which may function a mannequin for related establishments looking for to reinforce their an infection management measures.
Journal Reference
Youté OD, Domngang Noche C, Tamatcho Kweyang BP, Kougang EG and Fotsing Kwetche PR. “Floor decontamination effectiveness on the ‘Université des Montagnes’ Instructing Hospital: Monitoring within the biomedical evaluation laboratory” Heliyon, 2024; 10(4): e25647. DOI: https://doi.org/10.1016/j.heliyon.2024.e25647
In regards to the Authors
O’Neal Dorsel Youté , hospital hygiene and the technique of monitoring microbial presence within the hospital surroundings in resource-limited settings are amongst his analysis pursuits. The work offered on this platform is without doubt one of the outcomes of his closing grasp’s mission on the Université des Montagnes. Youté graduated in 2020 with a Grasp’s diploma in Medical Biology, specializing in Microbiology. He’s additionally fascinated by antimicrobial resistance and infectious illnesses points. (yout.oneal2@gmail.com)
Blandine Pulchérie Tamatcho Kweyang; I’m a Senior Lecturer within the Division of Microbiology, School of Sciences, College of Yaoundé I. Holder of a Ph.D/Doctorate in Animal Biology and Physiology, and specialised in Water and Environmental Microbiology, I give courses to Bachelor, Grasp and Doctorate college students in Soil and Surroundings. As researcher, the overall theme of our work issues 1- the identification of environmental micro organism that are etiologies of infectious illnesses, 2- the susceptibility profile of those micro organism to standard antibacterials brokers and, 3- the characterization of resistance genes. Microbial ecotoxicology constitutes the staff’s second analysis space (tamatcho@yahoo.com)
Esther Guladys Kougang; I maintain a Grasp’s diploma in Medical Microbiology and PhD scholar. I’m employed on the Nationwide Veterinary Laboratory (LANAVET), Cameroon. I’m concerned in hygiene in healthcare environments, surveillance and prognosis of animal and zoonotic illnesses utilizing molecular biology, serology, bacterial isolation, antibiotic resistance methods; in addition to within the high quality management of veterinary medicinal merchandise and foodstuffs by HPLC. (estherkougang@gmail.com)
Pierre René Fotsing Kwetché, Affiliate Professor, Medical and Environmental Microbiology
Microbiology laboratory Head, Researcher, and institutional Scholar One Well being Progressive Membership (SOHIC) Lead
Lecture in Bacteriology, Virology, Hospital Hygiene to Medical, Pharmacy, Dentistry, Veterinary and Laboratory Scientist Faculties. Analysis inclinations embody antimicrobial resistance (AMR) in hospitals, animal farms; Meals and Water high quality; Hospital Hygiene; Different Phytodrugs. ( prfotsingk@gmail.com /prfotsing@cum.aed-cm.org)
